Illumina reads for genome assembly of Pseudomonas amygdali 35-1 - SRA

SRX12312434: Illumina reads for genome assembly of Pseudomonas amygdali 35-1
1 ILLUMINA (Illumina MiSeq) run: 719,311 spots, 361.1M bases, 188.9Mb downloads

Design: High molecular weight genomic DNA was extracted (Miller et al. 1988) with the following modifications: the pre-lysozyme lysis buffer was 1M Tris pH 7.5, 0.5M EDTA pH 8.0, 5M NaCl, lysozyme digestion was performed at 48C for 1h, DNA resuspension was in 10 mM Tris pH 7.5, RNAse treatment was followed by two additional washes. DNA quantity was assessed using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, U.S.A.) and a Qubit 3.0 fluorometer (Thermo Fisher Scientific, U.S.A.). DNA integrity was assessed using the Agilent 2100 BioAnalyzer system (Agilent, U.S.A.). For short-read sequencing, the DNA library was prepared using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina with the NEBNext Multiplex Oligos (New England Biolabs, USA). AMPure XP beads (Beckman Coulter, USA) were used for DNA size-selection and purification. The DNA library was sequenced on a MiSeq platform (Illumina, USA). Further details will be presented in a future publication.

Submitted by: USDA Emerging Pest and Pathogen Research Unit (usdaeppru)

Study: Genome sequencing and assembly of Pseudomonas amygdali from hibiscus strain 35-1

PRJNA765326 • SRP338432 • All experiments • All runs

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The purpose of this project is to produce a high-quality genome assembly for Pseudomonas amygdali from hibiscus strain 35-1.

Sample: Microbe sample from Pseudomonas amygdali 35-1

SAMN21572715 • SRS10285771 • All experiments • All runs

Organism: Pseudomonas amygdali

Library:

Name: 35-1-illumina

Instrument: Illumina MiSeq

Strategy: WGS